Fig. 4

Immunolabelling of adventitious roots during development – AGP epitopes. a Primordium stage of AR, JIM8 epitope occurrence in ground and vascular tissue and in the layer between the AR and the scion (arrow); inset: absence of the JIM8 epitope during the initial stages of AR development (asterisk). b Magnification of a (asterisk)., Abundant occurrence of the JIM8 epitope in the scion cells (arrow); increase of the JIM8 presence in particular AR cells (open arrows), its absence from root cap and meristematic cells (asterisk). b’. Bright field of b. c Magnification of a (circled area), the JIM8 epitope is either localised in the wall (arrows), cytoplasm (open arrows) or is absent (asterisk), depending on the cell file. c’. Bright field of c. d Primordium stage of AR with the JIM13 epitope occurrence in the ground tissue and some root cap cells; inset: the JIM13 epitope present in the layer between the AR and the scion (arrow). e Magnification of d (asterisk). Tonoplast localisation of the JIM13 epitope (open arrows). f Punctate distribution of the JIM16 epitope in the cells of an AR root primordium (dotted line), no signal in scion tissues (asterisks); inset: the JIM16 epitope is present at the division stage of the initial cells with localisation in walls/plasmalemma (arrow) of the complex (outlined area). g the JIM16 epitope presence in the tonoplast (circled areas) and in the cytoplasm (open arrow) next to the vacuole (arrow);vacuole inset 1: bright field of g; inset 2: abundant JIM16 epitope occurrence in the root cap cells (open arrows) and in the meristematic cell files beneath (arrows), the right side of the dotted line. f – fibre, col – collenchyma, cor – cortex, xy – xylem vessels. Scale: 100 μm (a, a inset, d, f), 10 μm (b, b’, c, c’, d inset, e, f inset, g, g inset 1 and 2)