Table 2 Validation by QRT-PCR of differentially expressed genes
| Â | Â | Â | Array | Â | Â | Â | QRT-CR | Â | Â | Â |
|---|---|---|---|---|---|---|---|---|---|---|
| Â | Â | Â | cpfbp1 | Â | cyfbp | Â | cpfbp1 | Â | cyfbp | Â |
Gene | Putative function | Primer sequence | Rosette | Root | Rosette | Root | Rosette | Root | Rosette | Root |
At2g39730 | Rubisco activase | 5'ACGTCCAGCTCCCAGGAA 3'ACTCTCGCCCTCAAAGCA | 1.2 ±0.08 | 0.0 ±0.02 | 0.0 ±0.09 | 2.4 ±0.07 | 1.6 ±0.1 | 0.0 ±0.1 | -0.5 ±0.2 | 2.9 ±0.1 |
At3g19930 | sugar transporter 4 | 5' TATGTGGCGGCTTTGGTGTCTT 3' TTGGGCAAAGCCGTTGAAAGCA | 0.0 ±0.04 | 1.9 ±0.1 | 1.4 ±0.04 | 0.0 ±0.1 | -0.2 ±0.1 | 22.0 ±0.3 | 6.4 ±0.2 | 0.0 ±0.1 |
At1g67850 | unknown protein | 5'CTCCCTCCATGTGCTGCA 3'TTCCCGTCTGTCGCCTCA | 0.0 +-0.1 | 1.6 ±0.01 | 0.6 +-0.01 | 0.0 +-0.1 | 0.2 +-0.1 | 2.7 ±0.2 | -0.2 +-0.1 | 0.0 ±0.1 |
At2g20140 | 26S protease regulatory complex subunit 4 | 5'TGAGCCAGGCACTGGGAA 3'CGCTTGGTGCCAACAGCA | 0.7 ±0.1 | 0.0 ±0.02 | 0.0 ±0.03 | 2.2 ±0.08 | 1.2 ±0.1 | -0.2 ±0.1 | -0.8 ±0.2 | 2.7 ±0.2 |
At3g61850 | DAG1 (DOF affecting germination 1) | 5' ACCAACAACAACACACCGCA 3' TTTCTCTTGTGGCCTCGCCTTT | 2.8 ±0.01 | 0.0 ±0.01 | 1.2 ±0.1 | 2.6 ±0.01 | 3.1 ±0.2 | 0.0 ±0.1 | 2.3 ±0.2 | 2.6 ±0.2 |
At1g50460 | HKL1 hexokinase | 5'ACAGTTGTTGCGGTAGAAGGAGGT 3'AGAGCAGAGCCAATGCTAGAACCA | 0.0 ±0.01 | 0.0 ±0.01 | 0.0 ±0.01 | 3.7 ±0.02 | 0.0 ±0.1 | -0.9 ±0.1 | 0.0 ±0.1 | 31.6 ±0.3 |
At5g20340 | beta-1,3-glucanase 5 | 5' TTTGATGCGTTCGTGTGGGCAA 3' AACCCGTCGATGCCTTTGTT | 0.0 ±0.01 | 0.0 ±0.02 | 0.0 ±0.01 | 3.5 ±0.05 | 0.0 ±0.1 | 0.0 ±0.1 | 0.0 ±0.1 | 3.9 ±0.3 |
At1g28480 | GRX480 glutaredoxin protein | 5'ACGACAACCGTCGGGGAA 3'CGCCGCCTTGAACTCCAA | 2.5 ±0.01 | 0.0 ±0.01 | 0.0 ±0.01 | 0.0 ±0.01 | 3.1 ±0.2 | - | 0.5 ±0.3 | - |
At1g76760 | Thioredoxin AtY1 | 5' TGGTCCTTGCCAGTTCATGGTT 3' AGCACCCTCAAAGCGATCACAA | 0.0 ±0.02 | 0.0 ±0.02 | 4.0 ±0.1 | 0.0 ±0.01 | 0.0 ±0.1 | - | 4.9 ±0.2 | - |
At5g20830 | sucrose synthase 1 | 5' ACAGCCAACGTGAGCGTTTGAA 3' TGGCTTCAACCCTGGAAAGCAA | 2.3 ±0.1 | 0.0 ±0.02 | 0.0 ±0.07 | 0.0 ±0.02 | 3.0 ±0.2 | - | 0.5 ±0.1 | - |
At5g24780 | VSP1 (vegetative storage protein 1) | 5'TACGGTCTCCCACGTCCA 3'AAGGTGCCAGCTTCTGCA | -2.3 ±0.05 | 0.0 ±0.02 | 0.0 ±0.02 | 0.0 ±0.06 | -11.2 ±0.1 | - | 0.0 ±0.1 | - |
At1g07340 | ATSTP2 (sugar transporter 2) | 5' ATGGTGTGAACGCAATCGCT 3' AATACAATCAGCGGCACGGCAT | 0.0 ±0.02 | 0.0 ±0.01 | 2.8 ±0.05 | 0.0 ±0.05 | 0.5 ±0.1 | - | 2.9 ±0.2 | - |
At1g79530 | GAPCP-1; glyceraldehyde-3-phosphate dehydrogenase | 5' TGCAAGAAGTGTGCAACCCA 3' ATGTTGCAATGCGGAGGACCAA | 0.0 ±0.05 | 0.0 ±0.03 | 2.4 ±0.3 | 0.0 ±0.01 | 0.3 ±0.1 | - | 2.6 ±0.1 | - |
At4g15210 | beta-amylase | 5' AATGTGGTGGAAACGTTGGCGA 3' AACAGCGGTTCTTCCGGCAAAT | 0.0 ±0.01 | 0.0 ±0.02 | 2.3 ±0.01 | 0.0 ±0.06 | 0.3 ±0.1 | - | 2.4 ±0.1 | - |