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Fig. 6 | BMC Plant Biology

Fig. 6

From: OsACOS12, an orthologue of Arabidopsis acyl-CoA synthetase5, plays an important role in pollen exine formation and anther development in rice

Fig. 6

The OsACOS12 could partially restore acos5 fertility. a and b Structural representation of the proACOS5:OsACOS12 and the proOsACOS12:OsACOS12 constructs. c-f The main stem of the Col (c), acos5 (d), proACOS5:OsACOS12 with a acos5/acos5 background (e), and proOsACOS12:OsACOS12 with a acos5/acos5 background (f) plants. g-j Alexander staining of the anthers from the Col (g), acos5 (h), proACOS5:OsACOS12 (i), and proOsACOS12:OsACOS12 transgenic lines (j). Bars = 100 μm. k-n SEM examination of the dehiscent pollen grains of the wild type (k), acos5 (l), proACOS5:OsACOS12 (m), and proOsACOS12:OsACOS12 transgenic lines (n). Bars = 5 μm. o RT-PCR analysis of ACOS5 or OSACOS12 expression in the flower buds of the Col, acos5 plants and the proACOS5:OsACOS12 and proOsACOS12:OsACOS12 transgenic lines. TUBULIN was used to monitor the cDNA yield and integrity of the samples

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