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Table 5 qRT-PCR verification of sesame male-sterility / reproduction related 12 DEGs detected by RNA-seq

From: Comparative transcriptome profiling of the fertile and sterile flower buds of a dominant genic male sterile line in sesame (Sesamum indicum L.)

Query IDa

Subject IDa

E-valuea

Scorea

Description

Log2FCb

S/Fc

SIN_1007162

AT5G20710

0

1122

BGAL7; beta-galactosidase

2.31

5.87 ± 0.96

SIN_1021282

AT1G14750

4E-107

337

SOLO DANCER, cyclin

−2.67

0.52 ± 0.19

SIN_1016793

AT1G06260

2E-125

367

cysteine proteinase

4.47

20.16 ± 1.82

SIN_1004507

AT4G34850

0

624

chalcone and stilbene synthase

1.00

4.24 ± 2.36

SIN_1001388

AT4G21330

2E-36

129

DYT1, bHLH transcription factor

−2.07

0.48 ± 0.15

SIN_1003502

AT2G42940

1E-90

272

AT-hook DNA-binding protein

3.82

7.00 ± 1.23

SIN_1007695

AT2G19070

0

540

SHT

7.68

13.91 ± 1.03

SIN_1013713

AT3G27730

0

1274

ROCK-N-ROLLERS/AtMER3

−1.57

0.74 ± 0.15

SIN_1020712

AT4G29250

2E-141

416

acyl-transferase family protein

4.40

3.45 ± 4.02

SIN_1022113

AT3G57620

0

670

glyoxal oxidase-related protein

4.04

13.42 ± 0.58

SIN_1006818

AT4G20050

0

558

QRT3; polygalacturonase

1.97

4.34 ± 0.91

SIN_1019202

AT3G59530

0

608

strictosidine synthase

1.66

3.31 ± 0.57

  1. aThe homologue search using Blast; bRNA-seq Log2FC(S/F); cS/F means fold change of gene between sterile bud and fertile bud by qRT-PCR