The occurrence of spring forms in tetraploid Timopheevi wheat is associated with variation in the first intron of the VRN-A1 gene

Table 1 PCR markers for determining the presence of different alleles of VRN-A1, VRN-G1 in tetraploid Timopheevi wheats and diploid Ae. speltoides

PCR marker	Name	Primer (5’ → 3’)	Target allele(s)	Expected pro-duct size (bp)	Annealing temp. (°C)	Reference.
VRN-A1 marker^a	Vrn1AF	GAAAGGAAAAATTCTGCTCG	VRN-A1f	655	55	[13]
	Int1R	GCAGGAAATCGAAATCGAAG	VRN-A1f-del
			VRN-A1f-ins
			VRN-A1f-del/ins
VRN-A1 Non-deletion	Intr1/C/F	GCACTCCTAACCCACTAACC	VRN-A1f	1068	56	[14]
VRN-A1 Non-deletion	Intr1/AB/R	TCATCCATCATCAAGGCAAA	VRN-A1f-ins	1068	56	[14]
VRN-A1 insertion	Intr 1	ATCATCTTCTCCACCAAGGG	VRN-A1f	1480	50	[15]
	Intr1insR	AATGAACAGCACGGAAACAG	VRN-A1f-ins	1910
	Intr1insR	AATGAACAGCACGGAAACAG	VRN-A1f-del/ins
	mitef	CAGGTAAGGTATGAGGTGAC	VRN-A1f-ins	370	52	-
	miter	GATTCCAAATGAGAAGATGAGG	VRN-A1f-del/ins
VRN-A1 deletion of 2.7 kb	Intr1/A/F2	AGCCTCCACGGTTTGAAAGTAA	VRN-A1f-del	~2500	56	[14]
	Intr4	GCGCCATTAGGGAGGCACTT	VRN-A1f-del/ins
	delf	ACATGTAAGCAGATCCTATCGA	VRN-A1f-del	450	53	-
	delr	TGCTTTAGATCTTTCTTCACGG	VRN-A1f-del/ins
VRN-G1 marker^a
for T. araraticum/	P1	TACCCCTGCTACCAGTGCCT	VRN-G1	900	55	[23]
T. timopheevii	P5	GGCCAACCCTACACCCCAAG	VRN-G1a	1100
for Ae. speltoides	P8	CTAGGACTGGCGAGTATCTT	VRN-1	900	55	-
for Ae. speltoides	P10	GAGAACCGGGCCAACCCTAC
VRN-G1 Non-deletion
for T. araraticum/	Ex1/C/F	GTTCTCCACCGAGTCATGGT	VRN-G1	1530	56	[14]
T. timopheevii	Intr1/B/R4	CAAATGAAAAGGAATGAGAGCA	VRN-G1a
for Ae. speltoides	Ex1/C/F	GTTCTCCACCGAGTCATGGT	VRN-1	1530	60	[14]
for Ae. speltoides	Intr1/D/R4	AAATGAAAAGGAACGAGAGCG

^aThese diagnostic markers detect allelic variation at the promoter regions. In other cases variation within intron 1 of corresponding genes is detected

ISSN: 1471-2229