Fig. 5

Analysis of transcripts involved in the shikimate metabolic and indole/tryptophan pathways in Clematis terniflora DC. leaves by qRT-PCR pre- and post-treatment with high level of UV-B irradiation for 2 h (UV-B 2 h), 5 h (UV-B 5 h), 5 h followed by an incubation for 12 h in the dark (UV-B 5 h + Dark 12 h), and 5 h followed by an incubation for 36 h in the dark (UV-B 5 h + Dark 36 h). Abbreviations: SK, shikimate kinase; EPSPS, 5-enolpyruvylshikimate-3-phosphate synthase; CS, chorismate synthase; AS, anthranilate synthase; PAT, phosphoribosylanthranilate transferase; PAI, phosphoribosylanthranilate isomerase; IGPS, indole-3-glycerol phosphate synthase; TS, tryptophan synthase. Data are shown as the mean ± SD from three independent biological replicates. Asterisks indicate significant changes as measured by One-Way ANOVA test (*P < 0.05; **P < 0.01; ***P < 0.001)