Fig. 3

Gradients of GlnLux output of seedling leaves of maize seedlings using in situ imaging. Plants were initially treated with only water, and then at day 12, they were exposed to Hoagland’s nutrients solution containing either 0 mM N (−N) or 20 mM N (+N) for 1, 12, or 24 h, after which the leaves were harvested and placed on GlnLux agar. GlnLux images are shown directly beside light images of each leaf. Red-yellow-green indicates diminishing GlnLux response, and black indicates absence of GlnLux output. Three replicates of each treatment combination are displayed vertically