Fig. 1

Schematic images of the GlnLux protocols. Overview of the Glnlux liquid assay using extracts of leaf punches incubated with GlnLux biosensor cells in 96-well plates and measured using a luminometer (a). Overview of the GlnLux in situ imaging assay (b). Leaves are frozen at −80 °C and thawed at room temperature for 30 s to cause Gln leakage. Leaves are pressed down on agar pre-embedded with GlnLux cells, referred to as GlnLux agar. Plates are inverted and incubated for 2.5 h and then imaged for 1000 s using a luminescence imaging system. PIC, protease inhibition cocktail. Images are courtesy of Lisa Smith (University of Guelph), and can be re-used under the Creative Commons BY license