Fig. 4
From: Flavonoids and darkness lower PCD in senescing Vitis vinifera suspension cell cultures
Cytochrome c release in cytosolic fractions isolated from V. vinifera (cv. Limberger) suspension cell cultures. GSC and RSC were grown either under light or dark conditions for 6 days. Samples were obtained at day 0 and 6, respectively, for the analysis of cytochrome c release. The densitometric analysis of cross-reactivity signals were detected after Western blot of cytosolic proteins isolated from cell cultures, incubated with anti-cytochrome c primary antibody. Bars are means ± S.D. of at least three independent experiments. Different letters indicate significant difference (P ≤ 0.05), evaluated by ANOVA test