Fig. 3
From: Flavonoids and darkness lower PCD in senescing Vitis vinifera suspension cell cultures
PCD in V. vinifera (cv. Limberger) suspension cell cultures, evaluated by TUNEL assay. Cells at day 0 were observed under visible light (a, d); nuclei were stained with DAPI (b, e) or TMR-red for TUNEL assay (c, f), and observed under UV light, with low (b, c) and high (e, f) magnification (Panel a). TUNEL assay was performed in GSC and RSC grown under light or dark conditions at day 0 and 6, to evaluate the percentage of cells undergoing PCD (Panel b), counting the cells with red fluorescent-stained nuclei as apoptotic-like dead cells. Bars are means ± S.D. of at least three different experiments. Different letters indicate significant difference (P ≤ 0.05), evaluated by ANOVA test