BMC Plant Biology

Table 1 Summary of non-silent mutations in Psy1 identified by TILLING

From: Genetic analysis of phytoene synthase 1 (Psy1) gene function and regulation in common wheat

Gene	M3 Plant	Cultivar	Exon\Intron	Nucleotide change^a	Amino acid change^b	Codon change	Zygosity^c
Psy-A1	M091753	J22	Exon	C308T	A103V	GCA → GTA	Hom
	M090158 ^d	J20	Exon	C3201T	Q346*	CAG → TAG	Hom
	M092432	J20	Exon	C3255T	L364F	CTT → TTT	Hom
	M091887	J22	Exon	C335T	S112L	TCG → TTG	Hom
	M091949	J22	Exon	C349T	Q117*	CAG → TAG	Hom
	M090950	J22	Exon	G1224A	W172*	TGG → TAG	Hom
	M091151	J22	Exon	G1230A	R174K	AGG → AAG	Hom
	M090997	J22	Exon	G271A	E91K	GAG → AAG	Hom
	M092152	J22	Exon	G3231A	E356K	GAG → AAG	Het
	M091102	J22	Exon	G3554A	R397K	AGG → AAG	Hom
	M090333	J20	Exon	G3605A	G414E	GGG → GAG	Het
	M092889	J22	Exon	G371A	R124K	AGG → AAG	Hom
	M090755	J20	Exon	G400A	G134R	GGG → AGG	Hom
	M092383	J20	Exon	G412A	A138T	GCC → ACC	Het
	M091295	J22	Exon	G436A	E146K	GAG → AAG	Hom
	M092101	J22	Exon	G596A	E160K	GAG → AAG	Hom
	M090122	J20	Exon	G629A	V171G	GTA → AGT	Hom
	M092853	J22	Exon	T3169G	V335G	GTC → GGC	Het
Psy-B1	M091983	J22	Exon	G2073A	E244K	GAG → AAG	Het
Psy-D1	M092201	J20	Exon	C3792T	P370L	CCG → CTG	Het
	M091755	J22	Exon	C4109T	P409S	CCT → TCT	Hom
	M090628	J20	Exon	C4110T	P409L	CCT → CTT	Hom
	M091169	J22	Exon	G1347A	D217N	GAC → AAC	Het
	M091217	J22	Exon	G3609A	R309K	AGA → AAA	Hom
	M090649	J20	Exon	G3761A	V360M	GTG → ATG	Hom
	M090324	J20	Exon	G3779A	E366K	GAG → AAG	Het
	M091365	J22	Exon	G4049A	D389N	GAC → AAC	Het
	M092126	J22	Exon	G4071A	R396K	AGG → AAG	Hom
	M090608	J20	Exon	G4097A	V405M	GTG → ATG	Het

^athe first letter indicates the wild type nucleotide, the number is its position from the start codon, and the last letter is the mutant nucleotide
^bthe first letter indicates the wild type amino acid, the number is its position from the smethionine, and the last letter is the mutant amino acid
^cHom, homozygous genotype; Het, heterozygous genotype
^dbold items, mutations severely affecting phenotype
^e*, termination mutation

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ISSN: 1471-2229

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