Fig. 5

Impact of heat exposure on the expression of protein complexes in chloroplasts. a Correlation between protein production (RPF reads) and protein abundance determined by mass spectrometry [31]. Spearman coefficient ρ = 0.746. b The protein production of ATP synthetase subunits correlates with the assembled subunit stoichiometry. The genes belonging to each of the two operons are color-coded in blue and red. c Coverage plots for each of the subunits of the ATP synthetase complex under control growth. Note that the y-axes are in uniform scale. Schematic of the gene organization in the two operons is included over the plots. d Fold changes of RPF and mRNA of all chloroplast ORFs. Each dot represents a single protein or protein subunit. e Heat exposure disproportionately reduces the production of ATP synthetase subunits (compare with panel b)