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Fig. 5 | BMC Plant Biology

Fig. 5

From: Regulation of polar auxin transport in grapevine fruitlets (Vitis vinifera L.) and the proposed role of auxin homeostasis during fruit abscission

Fig. 5

Immunolocalization of putative VvPIN4 protein on longitudinal sections of 10 DAF grapevine fruitlets. a Detection of putative VvPIN4 protein in pericarp cells using anti-AtPIN4. b Control with anti-Actin showing diffuse not polarized fluorescence. Background fluorescence observed on sections treated with anti-AtPIN4 (c) and anti-Actin (d) preimmune serum instead of antiserum. Two independent immunolocalization assays with anti-AtPIN4 and anti-AtPIN4 preimmune serum are shown. Red fluorescence is emitted by FM 4-64 membrane stain. Green fluorescence is emitted by secondary antibody conjugated to fluorescent dye. Bars = 30 μm

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BMC Plant Biology

ISSN: 1471-2229

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