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Fig. 4 | BMC Plant Biology

Fig. 4

From: Characterization of eleven monosomic alien addition lines added from Gossypium anomalum to Gossypium hirsutum using improved GISH and SSR markers

Fig. 4

A set of G. anomalum-specific SSR markers were used to identify individual alien chromosomes of G. anomalum in G. hirsutum. a-k the G. anomalum-specific amplicons were obtained using 11 individual chromosome-specific primer pairs for markers; NAU5421, BNL2443, NAU7579, NAU3677, dPL0492, BNL2597, BNL3383, NAU4881, NAU9520, dPL0379, and dPL0864. The chromosomes correspond to D2 to D4 and D6 to D13 in cultivated tetraploid cotton. P1, G. hirsutum; P2, G. anomalum; F1, the hexaploid of G. hirsutum and G. anomalum; 1-11 show that each of these plants possesses a single different individual chromosome from G. anomalum, corresponding to 2Ba to 4Ba, and 6Ba to 13Ba. M, molecular size marker (50 bp ladder). Arrows (red) indicate chromosome-specific markers for G. anomalum

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