Fig. 3

Genomic in situ hybridization of the putative monosomic alien chromosomes of G. anomalum in the G. hirsutum background using G. herbaceum and G. anomalum probes. a mitotic chromosome spread of the 52 chromosomes of G. hirsutum, showing 26 chromosomes each of the At- (white) and Dt- (blue) subgenomes. b-l mitotic chromosome spread showing the 52 G. hirsutum (white and blue) chromosomes and different individual chromosomes from G. anomalum (red), corresponding to 2B^a to 4G^a (b, c and d) and 6G^a to 13G^a (e, f, g, h, i, j, k and l), respectively. Scale bar = 5μm