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Fig. 1 | BMC Plant Biology

Fig. 1

From: Characterization of eleven monosomic alien addition lines added from Gossypium anomalum to Gossypium hirsutum using improved GISH and SSR markers

Fig. 1

Genomic in situ hybridization of the putative alien chromosomes of G. anomalum in the G. hirsutum background using two G. herbaceum and G. anomalum probes. Genomic DNA from G. anomalum and G. herbaceum was labeled with digoxigenin-11-dUTP and Bio-16-dUTP by nick translation, respectively. Chromosomes of the At-subgenome of G. hirsutum were cross-hybridized with both the G. anomalum and G. herbaceum probes and produced white signals and chromosomes of the Dt-subgenome of G. hirsutum were stained with 4′,6-diamidino-2-phenylindole (DAPI) and produced blue signals. Chromosomes from G. anomalum were hybridized with G. anomalum probe and produced red signals. a mitotic chromosome spread of the 52 chromosomes of G. hirsutum. b mitotic chromosome spread of the 26 chromosomes of G. anomalum. cl mitotic chromosome spread showing the 52 G. hirsutum (white and blue) chromosomes and three (c), two (d), and one (e, f, g, h, i, j, k and l) individual chromosomes of G. anomalum (red), respectively. Scale bar = 5μm

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