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Table 2 Kinetic parameters for CYP79D60, CYP79D62, and CYP79D63. The maximal velocities were measured in the presence of 1 mM substrate. CYP79D63 showed no activity with L-Phe, L-Leu, L-Ile, and L-Tyr

From: CYP79D enzymes contribute to jasmonic acid-induced formation of aldoximes and other nitrogenous volatiles in two Erythroxylum species

  CYP79D60 CYP79D62 CYP79D63
substrate K m
(mM)
Maximal velocity
(ng*h-1*assay-1)
K m
(mM)
Maximal velocity
(ng*h-1*assay-1)
K m
(mM)
Maximal velocity
(ng*h-1*assay-1)
L-Phe 0.58 ± 0.05 160.44 ± 4.33 0.67 ± 0.07 424.00 ± 15.24 - -
L-Leu 0.23 ± 0.08 68.58 ± 2.49 0.59 ± 0.08 256.90 ± 7.45 - -
L-Ile 1.28 ± 0.25 32.62 ± 5.16 3.27 ± 0.42 42.55 ± 3.49 - -
L-Trp 2.74 ± 0.11 26.50 ± 0.32 1.09 ± 0.04 16.36 ± 0.31 0.48 ± 0.05 2.92 ± 0.14
L-Tyr 6.09 ± 1.32 4.66 ± 0.43 4.99 ± 0.46 73.18 ± 1.93 - -