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Table 2 Kinetic parameters for CYP79D60, CYP79D62, and CYP79D63. The maximal velocities were measured in the presence of 1 mM substrate. CYP79D63 showed no activity with L-Phe, L-Leu, L-Ile, and L-Tyr

From: CYP79D enzymes contribute to jasmonic acid-induced formation of aldoximes and other nitrogenous volatiles in two Erythroxylum species

 

CYP79D60

CYP79D62

CYP79D63

substrate

K m

(mM)

Maximal velocity

(ng*h-1*assay-1)

K m

(mM)

Maximal velocity

(ng*h-1*assay-1)

K m

(mM)

Maximal velocity

(ng*h-1*assay-1)

L-Phe

0.58 ± 0.05

160.44 ± 4.33

0.67 ± 0.07

424.00 ± 15.24

-

-

L-Leu

0.23 ± 0.08

68.58 ± 2.49

0.59 ± 0.08

256.90 ± 7.45

-

-

L-Ile

1.28 ± 0.25

32.62 ± 5.16

3.27 ± 0.42

42.55 ± 3.49

-

-

L-Trp

2.74 ± 0.11

26.50 ± 0.32

1.09 ± 0.04

16.36 ± 0.31

0.48 ± 0.05

2.92 ± 0.14

L-Tyr

6.09 ± 1.32

4.66 ± 0.43

4.99 ± 0.46

73.18 ± 1.93

-

-