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Fig. 7 | BMC Plant Biology

Fig. 7

From: Direct imaging of glycans in Arabidopsis roots via click labeling of metabolically incorporated azido-monosaccharides

Fig. 7

Optical sections of 4 day old Arabidopsis seedling roots incubated for 24 h with 25 μM acetylated Ac4GlcNAz (a), 100 μM Ac3ArabAz (b), 25 μM Ac4FucAz (c), 25 μM Ac4GalNAz (d) or 50 μM GlcNCyc (g) followed by labeling through strain-promoted alkyne-azide cycloaddition with DBCO-PEG4-ATTO-488 (a–d) or an inverse electron demand Diels-Alder click reaction with Tetrazine-ATTO-488 (g). As a control, seedlings were treated with 0.01 % DMSO followed by labeling through strain-promoted alkyne-azide cycloaddition with DBCO-PEG4-ATTO-488 (e) or an inverse electron demand Diels-Alder click reaction with Tetrazine-ATTO-488 (f). Seedling roots treated with DBCO-PEG4-ATTO-488 labeled Ac3ArabAz (100 μM, 24 h) (f, j) counterstained Propidium Iodide (PI, 0.05 %) to visualize cell walls (i, j). Yellow color indicates overlap of the two dyes (j). Scale bars = 25 μm (see Additional file 14 for high resolution)

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