Fig. 6

Course of total and single amino acids in leaf and stem base of Petunia hybrida cuttings exposed to three environment conditions – immediately planted for rooting, under dark exposure, planted for rooting after dark – and sampled at five exposition times (0, 6, 24, 72, 168 hpin or hpe, corresponding days are shown in the x-axis: 3d, 7d), respectively. Panels a to r: Full cycles and open squared symbols connected with full and intermittent lines represent leaf and steam base tissues, respectively. Total (free) amino acids are given as μmol g⁻¹ FM and single amino acids as nmol g⁻¹ FM, respectively. Multifactorial ANOVA’s revealed significant interaction effects for environment condition × exposition time × tissue type p < 0.00001 to p < 0.05 (Panels a-b-c, d-e-f, g-h-i, j-k-l, m-n-o, p-q-r). Vertical bars represent 95 % confidence intervals of mean values. Further details of experiments and statistics are presented in methods and with Additional file 1) Experiments of nitrogen preconditioning of cuttings, Additional file 2) Explanation of experimental designs for statistical analyses and Additional file 3) Supplemental data of figure 6 for Exp. 5: AA-DCR, respectively