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Table 2 Candidates of CDKA;1 substrates identified in this study

From: Modulation of plant growth in vivo and identification of kinase substrates using an analog-sensitive variant of CYCLIN-DEPENDENT KINASE A;1

Gene Locus

Identification

[R/K]xLa

[S/T]Pb

[S/T]Px[R/K]c

CYCD2d

PhosPhAte

At1g13440

GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE C2 (GAPC2)

2

–

–

n.d.

–

At1g35720

ANNEXIN 1 (ANNAT1)

4

–

–

n.d.

–

At1g52400

BETA GLUCOSIDASE 18 (BGLU18)

5

5

–

n.d.

–

At1g53240

MITOCHONDRIAL MALATE DEHYDROGENASE 1 (mMDH1)

1

2

–

+

–

At1g54100

ALDEHYDE DEHYDROGENASE 7B4 (ALDH7B4)

4

2

–

+

–

At2g14260

PROLINE IMINOPEPTIDASE (PIP)

3

1

–

+

–

At2g31390

pfkB-like carbohydrate kinase family protein

3

–

–

+

–

At2g36530

LOW EXPRESSION OF OSMOTICALLY RESPONSIVE GENES 2 (LOS2)

1

–

–

n.d.

–

At2g39730

RUBISCO ACTIVASE (RCA)

3

–

–

n.d.

–

At3g09440

Heat shock protein 70 (Hsp 70) family protein

1

2

–

n.d.

–

At3g09820

ADENOSINE KINASE 1 (ADK1)

3

2

–

n.d.

–

At3g51260

20S PROTEASOME ALPHA SUBUNIT PAD1 (PAD1)

2

2

–

n.d.

–

At3g52390

TatD related DNase

2

1

–

n.d.

–

At4g25240

SKU5 SIMILAR 1 (SKS1)

3

4

1

n.d.

–

At4g38220

AQUAPORIN INTERACTOR (AQI)

5

6

–

n.d.

–

At4g38970

FRUCTOSE-BISPHOSPHATE ALDOLASE 2 (FBA2)

2

4

1

–

2

At5g14200

ISOPROPYLMALATE DEHYDROGENASE 1 (IMD1)

2

2

1

+

1

At5g17920

METHIONINE SYNTHESIS 1 (ATMS1)

8

2

–

n.d.

1

At5g19440

NAD(P)-binding Rossmann-fold superfamily protein

4

2

–

n.d.

–

At5g26000

THIOGLUCOSIDE GLUCOHYDROLASE 1 (TGG1)

3

3

–

n.d.

–

At5g51830

pfkB-like carbohydrate kinase family protein

3

–

–

n.d.

–

At5g62790

1-DEOXY-D-XYLULOSE 5-PHOSPHATE REDUCTOISOMERASE (DXR)

2

2

–

n.d.

–

  1. aPutative cyclin binding motif; [RK].L. [0,1][FYLIVMP]
  2. bPutative minimal motif in CDK substrates at site of phosphorylation
  3. cPutative maximal motif in CDK substrates at site of phosphorylation
  4. dPhosphorylatin was confirmed by CDKA;1-CYCD2;1 complexes. +; positive -; negative n.d. not determined
  5. ePeptides phosphorylated at [S/T]P sites were found in the PhosPhAt 4.0 data base