Fig. 5

In vitro kinase assay against candidate proteins. a Histone H1 kinase assay. Proteins were subjected to SDS-PAGE after the kinase reaction with (+) or without (−) CDKA;1-CYCD2;1 complexes and stained with Coomassie brilliant blue R-250 demonstrating equal loading of the substrate. Abbreviations: p-histone H1 for radio-labeled histone H1 resulting from kinase assays with radio-labeled ATP. b In vitro kinase assays against candidate proteins. Proteins were subjected to SDS-PAGE after the kinase reaction with (+) or without (−) CDKA;1-CYCD2;1 complexes and stained with Coomassie brilliant blue R-250. Asterisks in the autoradiograph (top) show phosphorylated substrates, in the Coomassie stain (bottom) demonstrate equal loading of the recombinant substrate candidates, respectively. Abbreviations: p-CYCD2;1 for radio-labeled CYCD2;1 resulting from autophosphorylation by kinase assays with radio-labeled ATP