Fig. 7

Dominant chimeric repressor-mediated suppression of ONAC095 function increased ABA sensitivity in ONAC095-SRDX lines. a and b Germination performance and rates of ONAC095-OE, ONAC095-SRDX and WT seeds on 1/2 MS medium supplemented with or without 5 μM ABA. c–f Growth performance of ONAC095-OE, ONAC095-SRDX and WT seedlings grown on 1/2 MS medium with or without 5 μM ABA. Weight of single seedling (d) and length of shoot (e) and root (f) were measured at 6 days after germination. g ABA content in two-week-old ONAC095-OE, ONAC095-SRDX and WT plants grown under normally watered condition. h Expression levels of ABA biosynthesis- and metabolism-related and ABA signaling-related genes in ONAC095-SRDX and WT plants. Relative expression levels were normalized by the transcript level of the Actin gene as an internal control and the expression level of the tested genes in WT plants under normal condition was set as 1. Data presented in (b) and (d–h) are the means ± SD from three independent experiments and columns with an asterisk indicate significant difference at p < 0.05 level between WT and OE/SRDX lines. WT, wild type; OE6, ONAC095-OE6; OE12, ONAC095-OE12; S2, ONAC095-SRDX2; S3, ONAC095-SRDX3