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Fig. 5 | BMC Plant Biology

Fig. 5

From: Rice NAC transcription factor ONAC095 plays opposite roles in drought and cold stress tolerance

Fig. 5

Dominant chimeric repressor-mediated suppression of ONAC095 function in ONAC095-SRDX plants attenuated cold stress tolerance but did not affect heat stress tolerance. a Growth performance of ONAC095-OE, ONAC095-SRDX and WT plants at different stages during a cold stress experiment. ONAC095-OE and ONAC095-SRDX plants were grown in same barrels with WT and were subjected to cold stress treatment in a growth chamber with temperature at 4 °C for 5 days for ONAC095-OE/WT plants or for 1 day for ONAC095-SRDX/WT plants. The cold stressed plants were recovered under normal condition for 7 days. b Survival rates of the cold stress-treated plants. ce Comparison of MDA content (c), electrolyte leakage (d) and chlorophyll content (e) in leaves of ONAC095-SRDX and WT plants with or without cold treatment. f Expression of cold stress-related genes in ONAC095-SRDX and WT plants before and after 1 day of cold treatment. Relative expression levels were normalized by the transcript level of the Actin gene as an internal control and the expression level of the tested genes in WT plants under normal condition was set as 1. g Survival rates of the heat stress-treated plants. Three-week-old WT, ONAC095-OE and ONAC095-SRDX plants were treated with heat stress in a growth chamber with temperature of 45 °C for 5 days and survivals were recorded at 7 days after recovering under normal condition. Data presented in (bg) are the means ± SD from three independent experiments and columns with an asterisk indicate significant difference at p < 0.05 level between WT and SRDX lines. WT, wild type; OE6, ONAC095-OE6; OE12, ONAC095-OE12; S2, ONAC095-SRDX2; S3, ONAC095-SRDX3

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