Table 2 Nucleotide sequences of primer pairs designed for chromosome specific alleles of GBSSI and isoforms of SBEII for quantitative real time PCR
Gene | NCBI ID | Forward primer (5’-3’) | Tm (°C) | Reverse primer (5’-3’) | Tm (°C) | Amplicon size (bp) |
|---|---|---|---|---|---|---|
GBSSI-7A | EU719608.1 | GAATGCGCTACGGAACGCCG | 57.9 | CCGCCTCAGCGTTGACTGCAA | 58.3 | 109 |
GBSSI-4A | EU719610.1 | TCGGCACGCCAGCCTACCAT | 57.9 | GGGTCATCGGCGAGGAGATT | 55.9 | 143 |
GBSSI-7D | EU719612.1 | GACAATAACCCCTACTTTTCTGGG | 55.7 | CAGGGCCGCAAAGGTGGCAT | 57.9 | 139 |
SBEIIa | AF338432.1 | GAACCGACTCAAGGCATTGTGG | 56.7 | CGGAGCCATCTTGACTACC | 53.2 | 166 |
SBEIIb | AY740401.1 | CAGTCGCCATCGCTGCTCTTC | 58.3 | ATGATCCCTGACGGCGGTAG | 55.9 | 137 |