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Table 1 Evaluation of amylose content, resistant starch content, and thousand kernel weight (TKW) in the 101 EMS treated M4 mutant lines

From: Development of EMS-induced mutation population for amylose and resistant starch variation in bread wheat (Triticum aestivum) and identification of candidate genes responsible for amylose variation

Mutant lines

Amylose Content (AC)

Resistant Starch (RS)

TKW

Amylose Content (AC)

Resistant Starch (RS)

TKW

Biological replication # 1

Biological replication # 2

‘C 306’ (parent)

26.2 ± 0.4

00.8 ± 0.1

40.6 ± 0.14

26.6 ± 0.0

00.5 ± 0.5

40.3 ± 0.4

HAM (66 %)

65.5 ± 0.4

36.8 ± 0.0

-

65.5 ± 0.4

36.9 ± 1.1

-

TAC 6

06.8 ± 0.0

00.2 ± 0.3

46.0 ± 0.21

06.6 ± 0.1

00.8 ± 1.2

45.9 ± 0.1

TAC 28

73.2 ± 0.4

39.6 ± 1.2

48.1 ± 0.57

73.1 ± 0.3

45.3 ± 0.1

48.0 ± 0.4

TAC 35

68.7 ± 0.4

30.2 ± 0.2

41.2 ± 1.06

68.5 ± 0.3

28.4 ± 0.5

40.9 ± 0.6

TAC 51

67.0 ± 0.2

30.4 ± 1.1

39.2 ± 0.42

67.1 ± 0.2

30.7 ± 0.4

39.2 ± 0.5

TAC 71

68.7 ± 0.1

32.4 ± 0.1

43.2 ± 0.42

68.6 ± 0.4

34.9 ± 0.8

42.9 ± 0.1

TAC 74

69.2 ± 0.5

35.4 ± 0.7

45.7 ± 0.35

69.0 ± 0.3

37.4 ± 2.0

51.4 ± 8.3

TAC 75

64.4 ± 0.4

37.8 ± 1.1

49.0 ± 0.28

64.2 ± 0.5

35.8 ± 0.2

49.2 ± 0.5

TAC 104

04.4 ± 0.6

00.0 ± 0.0

47.2 ± 0.42

04.6 ± 0.1

00.0 ± 0.0

47.4 ± 0.7

TAC 137

18.5 ± 0.3

00.7 ± 0.0

45.8 ± 0.28

18.6 ± 0.1

01.2 ± 0.7

45.8 ± 0.3

TAC 163

16.3 ± 0.5

00.3 ± 0.0

42.8 ± 0.57

16.1 ± 0.1

00.5 ± 0.0

43.2 ± 1.0

TAC 176

13.8 ± 0.2

00.1 ± 1.1

44.0 ± 0.42

13.6 ± 0.2

02.1 ± 0.2

43.9 ± 0.2

TAC 197

24.8 ± 0.3

00.4 ± 0.3

44.8 ± 0.28

25.1 ± 0.2

00.4 ± 0.3

45.2 ± 1.0

TAC 237

07.7 ± 0.3

00.1 ± 0.7

40.7 ± 0.78

07.5 ± 0.2

02.2 ± 0.1

40.6 ± 0.5

TAC 243

43.6 ± 0.3

10.0 ± 0.9

47.6 ± 0.42

43.7 ± 0.1

10.0 ± 0.9

47.9 ± 0.9

TAC 273

25.9 ± 0.1

00.9 ± 0.8

47.7 ± 0.42

25.7 ± 0.1

02.2 ± 0.1

48.0 ± 0.8

TAC 287

35.7 ± 0.2

01.2 ± 1.2

43.6 ± 0.49

35.5 ± 0.2

00.4 ± 0.1

43.9 ± 0.9

TAC 288

11.6 ± 0.5

00.3 ± 0.5

41.7 ± 0.35

11.8 ± 0.2

00.3 ± 0.5

42.2 ± 0.9

TAC 308

37.4 ± 0.3

02.0 ± 1.7

45.9 ± 0.57

36.9 ± 0.3

04.5 ± 0.3

46.2 ± 1.1

TAC 354

20.4 ± 0.7

00.5 ± 0.2

32.8 ± 0.35

20.2 ± 0.1

01.0 ± 0.4

34.1 ± 2.1

TAC 358

02.6 ± 0.5

00.1 ± 0.2

42.2 ± 0.99

02.9 ± 0.3

00.3 ± 0.0

42.1 ± 0.9

TAC 360

46.4 ± 0.2

12.1 ± 1.4

43.0 ± 0.78

46.7 ± 0.3

12.3 ± 1.5

42.7 ± 0.4

TAC 362

35.9 ± 0.6

02.6 ± 0.7

41.7 ± 0.71

36.2 ± 0.4

03.6 ± 0.6

41.2 ± 0.3

TAC 369

39.3 ± 0.5

07.6 ± 0.7

45.4 ± 0.35

39.1 ± 0.2

10.0 ± 1.3

45.6 ± 0.5

TAC 374

14.6 ± 0.6

00.0 ± 0.1

46.7 ± 0.49

14.6 ± 0.1

00.0 ± 0.1

47.5 ± 0.9

TAC 380

26.3 ± 0.3

01.3 ± 1.3

41.0 ± 0.21

26.0 ± 0.3

02.8 ± 0.7

41.0 ± 0.1

TAC 381

29.2 ± 0.2

01.7 ± 1.1

45.2 ± 0.07

29.5 ± 0.0

03.2 ± 0.9

45.8 ± 0.8

TAC 399

75.7 ± 0.4

41.3 ± 0.1

39.2 ± 0.49

76.0 ± 0.4

42.6 ± 0.1

39.5 ± 0.8

TAC 404

46.8 ± 0.5

14.7 ± 1.4

46.0 ± 0.71

46.4 ± 0.1

16.2 ± 0.6

46.2 ± 0.9

TAC 418

35.3 ± 0.5

01.5 ± 0.1

48.2 ± 0.07

35.0 ± 0.1

01.5 ± 0.6

48.7 ± 0.7

TAC 419

32.0 ± 0.3

01.2 ± 0.0

46.5 ± 0.49

32.5 ± 0.1

01.2 ± 0.0

46.4 ± 0.2

TAC 421

36.4 ± 0.2

01.5 ± 0.3

32.9 ± 0.57

36.3 ± 0.0

02.0 ± 1.0

32.7 ± 0.4

TAC 423

20.0 ± 0.2

00.0 ± 1.1

46.7 ± 0.28

19.8 ± 0.2

01.5 ± 1.9

46.9 ± 0.6

TAC 428

43.4 ± 0.2

12.7 ± 0.8

37.6 ± 0.64

43.9 ± 0.1

07.7 ± 0.8

37.6 ± 0.6

TAC 437

51.5 ± 0.3

14.4 ± 1.2

43.9 ± 0.35

51.5 ± 0.1

14.5 ± 1.9

44.2 ± 0.7

TAC 457

34.9 ± 0.2

01.3 ± 0.6

41.9 ± 0.35

34.4 ± 0.0

02.8 ± 1.5

41.8 ± 0.3

TAC 477

35.5 ± 0.3

02.3 ± 1.4

45.6 ± 0.64

35.6 ± 0.1

06.3 ± 1.3

45.4 ± 0.3

TAC 536

16.4 ± 0.1

00.3 ± 0.3

51.2 ± 0.35

16.3 ± 0.0

02.8 ± 1.7

50.7 ± 1.0

TAC 539

16.1 ± 0.2

00.0 ± 0.1

48.1 ± 0.28

16.1 ± 0.0

00.5 ± 0.4

49.0 ± 0.9

TAC 560

19.7 ± 0.0

00.7 ± 0.0

46.2 ± 0.35

19.6 ± 0.1

01.2 ± 0.7

46.8 ± 0.6

TAC 587

13.1 ± 0.3

00.5 ± 0.2

41.1 ± 0.35

13.2 ± 0.0

01.5 ± 1.6

42.0 ± 0.8

TAC 606

43.2 ± 0.0

10.2 ± 0.1

48.1 ± 0.14

42.5 ± 0.1

09.7 ± 0.5

48.5 ± 0.7

TAC 622

42.7 ± 0.2

09.7 ± 0.4

39.1 ± 0.14

40.8 ± 0.0

00.2 ± 0.2

40.6 ± 2.0

TAC 623

20.9 ± 0.4

00.2 ± 0.1

46.5 ± 0.57

20.8 ± 0.0

08.4 ± 0.5

46.4 ± 0.6

TAC 636

42.0 ± 0.2

14.7 ± 0.2

42.6 ± 0.42

42.6 ± 0.0

13.7 ± 1.1

43.3 ± 0.7

TAC 662

44.4 ± 0.5

15.6 ± 0.9

52.1 ± 0.21

44.8 ± 0.0

02.1 ± 0.2

52.6 ± 0.5

TAC 681

26.9 ± 0.7

00.8 ± 1.5

48.8 ± 0.14

26.8 ± 0.1

09.6 ± 1.0

48.9 ± 0.2

TAC 696

46.8 ± 0.3

15.4 ± 0.1

39.2 ± 0.35

43.7 ± 0.1

06.4 ± 1.6

39.6 ± 0.9

TAC 703

15.2 ± 0.2

00.4 ± 0.0

49.4 ± 0.28

17.8 ± 0.1

00.2 ± 0.1

49.6 ± 0.6

TAC 14

63.4 ± 0.6

23.4 ± 1.1

41.1 ± 0.21

63.4 ± 0.0

22.4 ± 0.2

45.7 ± 0.7

TAC 708

23.8 ± 0.2

00.5 ± 0.3

45.4 ± 0.28

23.7 ± 0.1

01.2 ± 0.3

47.8 ± 1.0

TAC 711

17.9 ± 0.2

00.1 ± 0.2

47.5 ± 0.57

17.8 ± 0.1

00.1 ± 0.2

46.0 ± 1.0

TAC 713

13.4 ± 0.0

00.3 ± 0.7

43.3 ± 0.00

13.4 ± 0.0

02.0 ± 0.6

43.7 ± 0.5

TAC 730

11.2 ± 0.3

00.2 ± 0.1

47.7 ± 0.21

11.2 ± 0.0

02.3 ± 2.2

46.8 ± 1.5

TAC 737

13.0 ± 0.5

00.2 ± 0.3

43.4 ± 0.14

12.9 ± 0.1

00.4 ± 0.1

43.7 ± 0.4

TAC 741

18.5 ± 0.4

00.3 ± 0.0

46.7 ± 0.21

18.3 ± 0.3

00.8 ± 0.7

46.5 ± 0.2

TAC 747

24.5 ± 0.3

00.5 ± 0.5

47.2 ± 0.14

24.5 ± 0.1

00.0 ± 0.1

47.6 ± 0.5

TAC 748

25.7 ± 0.4

00.1 ± 0.2

44.5 ± 0.07

25.4 ± 0.3

00.1 ± 0.2

45.0 ± 0.8

TAC 765

15.9 ± 0.3

00.3 ± 0.9

40.5 ± 0.49

15.7 ± 0.2

01.6 ± 0.9

40.4 ± 0.2

TAC 766

19.3 ± 0.3

00.5 ± 0.7

49.6 ± 0.49

19.5 ± 0.1

00.5 ± 0.7

49.7 ± 0.6

TAC 781

29.4 ± 0.0

00.7 ± 2.9

42.4 ± 0.21

29.4 ± 0.0

03.2 ± 0.8

42.7 ± 0.5

TAC 790

40.9 ± 0.5

09.7 ± 0.5

41.6 ± 0.35

40.7 ± 0.2

09.7 ± 0.5

41.7 ± 0.4

TAC 810

19.1 ± 0.0

00.4 ± 0.3

46.4 ± 0.57

19.0 ± 0.0

00.9 ± 0.3

47.2 ± 0.6

TAC 824

49.1 ± 0.4

14.2 ± 0.2

38.1 ± 0.49

49.2 ± 0.1

14.7 ± 0.9

38.9 ± 1.6

TAC 831

33.2 ± 0.2

01.7 ± 0.0

48.7 ± 0.28

33.3 ± 0.1

05.2 ± 0.7

48.9 ± 0.6

TAC 846

07.1 ± 0.2

00.3 ± 0.5

50.6 ± 0.57

07.1 ± 0.1

00.3 ± 0.5

50.6 ± 0.6

TAC 869

26.9 ± 0.2

00.9 ± 0.4

42.7 ± 0.42

26.8 ± 0.1

01.9 ± 0.9

43.0 ± 0.8

TAC 880

12.4 ± 0.2

00.2 ± 0.1

45.4 ± 0.64

12.5 ± 0.1

00.0 ± 0.0

45.5 ± 0.7

TAC 902

20.4 ± 0.2

00.5 ± 0 .2

45.1 ± 0.21

20.5 ± 0.1

01.0 ± 0.4

46.2 ± 1.4

TAC 903

29.3 ± 0.2

01.0 ± 0.2

43.7 ± 0.64

29.5 ± 0.1

01.4 ± 0.8

43.7 ± 0.5

TAC 914

17.5 ± 0.3

00.2 ± 0.1

40.9 ± 0.14

17.7 ± 0.2

00.2 ± 0.1

41.2 ± 0.5

TAC 917

27.1 ± 0.3

00.5 ± 1.8

48.2 ± 0.57

26.9 ± 0.2

01.5 ± 1.8

47.9 ± 0.2

TAC 942

33.8 ± 0.1

01.2 ± 1.4

45.1 ± 0.14

33.8 ± 0.1

02.2 ± 0.0

45.6 ± 0.9

TAC 947

50.8 ± 0.4

12.0 ± 0.6

42.1 ± 0.14

50.6 ± 0.1

13.0 ± 0.7

42.9 ± 0.9

TAC 955

26.5 ± 0.2

00.5 ± 0.2

40.0 ± 0.42

26.6 ± 0.1

01.5 ± 1.6

39.9 ± 0.3

TAC 975

55.1 ± 0.2

19.7 ± 14.

47.7 ± 0.35

55.2 ± 0.0

20.2 ± 8.4

48.0 ± 0.7

TAC 981

11.6 ± 0.3

00.1 ± 0.9

45.5 ± 0.49

11.4 ± 0.2

02.1 ± 0.2

45.6 ± 0.5

TAC 989

32.0 ± 0.5

01.3 ± 1.4

43.4 ± 0.35

31.8 ± 0.2

02.8 ± 0.7

43.9 ± 0.9

TAC 990

25.5 ± 0.1

00.8 ± 1.7

35.3 ± 0.35

25.4 ± 0.1

02.8 ± 0.3

37.3 ± 2.4

TAC 1024

51.9 ± 0.3

12.6 ± 2.3

29.9 ± 0.85

51.2 ± 0.2

11.6 ± 0.9

32.1 ± 4.1

TAC 1025

11.6 ± 0.5

00.2 ± 0.9

49.0 ± 0.07

11.8 ± 0.3

01.4 ± 0.2

49.7 ± 0.9

TAC 1026

12.6 ± 0.2

00.0 ± 0.1

40.7 ± 0.42

12.5 ± 0.1

00.0 ± 0.1

40.8 ± 0.5

TAC 1046

16.1 ± 0.4

00.2 ± 0.0

44.9 ± 0.92

15.8 ± 0.3

01.2 ± 1.3

44.9 ± 0.8

TAC 1054

14.6 ± 0.3

00.3 ± 0.0

42.9 ± 0.99

14.4 ± 0.2

01.4 ± 0.7

42.9 ± 1.0

TAC 1068

35.9 ± 0.2

02.1 ± 0.0

37.2 ± 0.85

21.1 ± 0.3

02.1 ± 0.0

37.8 ± 1.7

TAC 1072

37.3 ± 0.1

02.7 ± 0.2

37.4 ± 0.64

40.0 ± 0.0

03.7 ± 0.2

38.4 ± 0.7

TAC 1075

21.2 ± 0.2

00.6 ± 0.5

39.0 ± 0.71

46.3 ± 0.2

00.8 ± 0.5

40.0 ± 0.7

TAC 1081

12.8 ± 0.2

00.0 ± 2.9

45.6 ± 0.49

46.7 ± 0.2

00.8 ± 1.4

45.8 ± 0.7

TAC 1090

57.1 ± 0.2

19.9 ± 3.4

32.5 ± 0.71

47.6 ± 0.1

16.9 ± 0.7

32.0 ± 0.0

TAC 1151

52.6 ± 0.5

14.8 ± 0.6

46.8 ± 0.42

7.3 ± 0.0

15.8 ± 0.6

47.0 ± 0.7

TAC 1168

49.2 ± 0.0

21.0 ± 2.0

44.1 ± 0.64

50.0 ± 0.1

18.5 ± 1.4

44.1 ± 0.5

TAC 1171

63.4 ± 0.6

37.6 ± 3.3

46.2 ± 0.49

36.0 ± 0.1

06.1 ± 1.1

46.4 ± 0.8

TAC 1193

73.2 ± 0.4

40.9 ± 1.5

41.1 ± 0.21

73.4 ± 0.1

42.4 ± 0.8

41.8 ± 0.7

TAC 1194

68.7 ± 0.4

36.5 ± 0.2

47.4 ± 0.35

21.0 ± 0.1

35.0 ± 0.4

48.2 ± 0.6

TAC 1201

67.0 ± 0.2

36.6 ± 0.1

40.9 ± 0.57

12.9 ± 0.1

34.6 ± 0.1

40.8 ± 0.3

TAC 1202

68.7 ± 0.1

36.2 ± 0.5

39.2 ± 0.49

57.2 ± 0.1

33.2 ± 1.9

38.9 ± 0.1

TAC 1207

35.7 ± 0.2

02.6 ± 1.7

42.7 ± 0.28

52.4 ± 0.2

03.6 ± 1.7

43.9 ± 1.5

TAC 364

40.2 ± 0.5

03.8 ± 1.8

60.0 ± 0.14

40.2 ± 0.1

03.3 ± 1.1

58.0 ± 2.8

TAC 172

30.1 ± 0.3

01.5 ± 1.6

60.0 ± 1.20

30.0 ± 0.0

02.0 ± 0.9

59.5 ± 2.1

TAC 988

23.8 ± 0.2

00.6 ± 0.4

62.0 ± 0.99

23.7 ± 0.1

01.1 ± 1.1

61.4 ± 1.9

TAC 1105

24.7 ± 1.0

00.6 ± 0.1

50.6 ± 0.21

24.7 ± 0.0

01.1 ± 0.8

50.9 ± 0.1

  1. Amylose content was measured by Concanavalin A (Con A) method in seed starch. Resistant starch content was measured through a modified protocol of Megazyme. Thousand kernel weight (grams) was recorded on randomly selected seeds