Skip to main content

Table 3 Peak data for AP2 phosphorylation

From: Kinase-Associated Phosphoisoform Assay: a novel candidate-based method to detect specific kinase-substrate phosphorylation interactions in vivo

pI Area %: AP2 +
- flg MPK6 + flg MPK3 + flg MPK1+ flg
4.76 0 0 2.32 0 0
4.81 0 0 0 0.65 0
4.88 0 0 2.27 0.49 0
4.94 0 0 0 3.6 1.62
5.01 0.27 2.32 21.96 8.4 4.49
5.09 7.56 27.44 6.88 16.65 10.69
5.15 0 0 18.03 0 0
5.17 26.23 0 0 0 18.92
5.19 0 16.78 0 23.94 0
5.24 36.49 27.17 29.85 12.42 26.16
5.29 0 0 0 0 14.26
5.32 14.59 0 0 0 0
5.36 0 6.37 0 10.31 6.49
5.43 0 3.81 0 0 7.49
5.46 0 0 18.69 7.94 0
5.49 4.84 0 0 0 0
5.57 10.04 16.1 0 15.58 9.87
  1. Data correspond to the electropherograms shown in Fig. 5a. Peaks 5.15-5.19 may represent the same isoform, not well separated from the neighbouring major peaks. Accumulation of a major isoform of pI ~ 5.0 occurred exclusively in the MPK6 + flg22 samples in three independent biological repeats