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Fig. 5 | BMC Plant Biology

Fig. 5

From: Kinase-Associated Phosphoisoform Assay: a novel candidate-based method to detect specific kinase-substrate phosphorylation interactions in vivo

Fig. 5

AP2 is an MPK6 substrate in vivo. a Effect of MAPK co-expression and flg treatment on C-terminal myc-tagged AP2 isoform distributions in cIEF-immunoassay. Expressed proteins and treatments are indicated for each sample. Dashed line indicates the position of a major acidic isoform specifically appearing in the presence of activated MPK6. b Conventional SDS-PAGE immunoblot of transiently expressed AP2:myc variants in the samples corresponding to panel a. Negative control (neg. cont.) denotes a protoplast sample not transfected with the myc epitope

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BMC Plant Biology

ISSN: 1471-2229

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