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Fig. 4 | BMC Plant Biology

Fig. 4

From: Kinase-Associated Phosphoisoform Assay: a novel candidate-based method to detect specific kinase-substrate phosphorylation interactions in vivo

Fig. 4

WUS is an MPK3 substrate in vitro. Kinase assay with in vitro translated, affinity purified wild-type GST-WUS (WUS) and T108A, S112A mutant GST-WUS (WUS-AA) variants. C: control, MPK3: WUS variants incubated with in vitro translated, affinity purified, eluted MKK4/MPK3. SDS-PAGE separated WUS protein on the autoradiograph is indicated by arrowhead. Phosphorylation of myelin basic protein (MBP) by MPK3 is shown in the right panel

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BMC Plant Biology

ISSN: 1471-2229

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