Fig. 1

DAG1 expression profile is controlled at epigenetic level. a Relative expression level of DAG1 in wild type (WT) developing seeds at 13, 16 and 19 days after pollination (DAP), and in mature dry seeds at 0 and 28 days after harvest (DAH). b Relative expression level of DAG1 in WT seeds, after 0, 6, 12 and 24 h imbibition. The values of relative expression levels are the mean of three biological replicates, presented with SD values. Significant differences were analyzed by t-test (*P ≤ 0,05). Expression levels were normalized with that of the UBQ10 (At4g05320) reference gene. c Graphic representation of the DAG1 locus. Above thick lines marked by numbers (1, 2, 3) are referred to different regions used for qPCR, corresponding to DAG1 promoter (1), 5′end (2) and coding sequence (3) respectively. d and e Chromatin from WT developing seeds at 10/13 DAP, 0 DAH, 24 h-imbibed seeds, and 14 days-old seedlings was immunoprecipitated with specific antibodies against the H3K27me3 (d), or the H3K4me3 (e) epigenetic marks. The amount of DNA was measured by qPCR. The values of fold enrichment were normalized to internal controls (relative to input and to PP2A), and are the average of three biological replicates presented with SD values. Significant differences were analyzed by t-test (*P ≤ 0,05), and calculated with respect to 0 DAH