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Fig. 3 | BMC Plant Biology

Fig. 3

From: Hydrogen cyanamide breaks grapevine bud dormancy in the summer through transient activation of gene expression and accumulation of reactive oxygen and nitrogen species

Fig. 3

In situ detection of H2O2, O2 •- and NO at early stages of grapevine budbreak. Visualization of H2O2 by fluorescence microscopy using DCF-DA assay in grapevine bud sections after 12 h of treatment: H2O as a control (a), P (b), HC (c) and PHC (d). For negative control, grapevine bud sections were incubated with 1 mM sodium pyruvate, an H2O2 scavenger: control (e), P (f), HC (g) and PHC (h). Visualization of O2 •- by reaction with 10 μM dihydroethidium (DHE) in grapevine bud sections: control (i), P (j), HC (k) and PHC (l). For negative control, grapevine bud sections were incubated in 1 mM tetramethylpiperdinooxy, an O2 •- scavenger: control (m), P (n), HC (o) and PHC (p). Visualization of nitric oxide (NO) by DAF-2DA assay in grapevine bud sections: control (q), P (r), HC (s) and PHC (t). For negative control, grapevine bud sections were incubated in 10 μM carboxy-PTIO, an NO inhibitor: control (u), P (v), HC (w) and PHC (x). Bar: 200 μm

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BMC Plant Biology

ISSN: 1471-2229

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