Skip to main content
Fig. 2 | BMC Plant Biology

Fig. 2

From: Calcium/calmodulin alleviates substrate inhibition in a strawberry UDP-glucosyltransferase involved in fruit anthocyanin biosynthesis

Fig. 2

Purification and glycosylation activity analysis of recombinant FvUGT1. a. SDS-PAGE and Western blotting showing the purity of the recombinant FvUGT1. The SDS-PAGE gel was stained with Coomassie Blue (left panel). Western blotting was performed against an anti-His-tag antibody (right panel). T, total cell lysate; P, pellets; S, supernatant; F, flow-through; L, last wash; E1, 2, 3, 4, the first, second, third and fourth elution. b, c, d. HPLC chromatograms of pelargonidin (substrate), reaction mix of FvUGT1 glycosylation, and pelargonidin-3-O-glucoside (product). mAU, milli-absorbance unit. e. Overlaid UV absorption spectra of pelargonidin (substrate) and pelargonidin-3-O-glucoside (product)

Back to article page