Cell wall dynamics during apple development and storage involves hemicellulose modifications and related expressed genes

Table 2 Soluble acidic and neutral sugars content released by sequential treatment of AIM

Treatment		60DAF		110DAF		H		1 M		2 M		Stat
WS	NSP	1.8	±0.1	2.7	±0.2	2.2	±0.1	1.8	±0.2	2.2	±0.2	c
	UA	0.5	±0.1	1.2	±0.1	0.9	±0.2	0.9	±0.1	1.3	±0.1	abc
	NS	1.3	±0.2	1.4	±0.2	1.3	±0.2	0.9	±0.1	0.9	±0.2	bc
PS	NSP	25.3	±0.6	27.3	±2.7	19.3	±1.2	16.4	±1.3	15.2	±0.9	abc
	UA	5.0	±0.6	5.3	±1.1	4.9	±0.5	4.7	±0.5	4.3	±0.2
	NS	20.4	±1.8	22.0	±6.2	14.4	±0.9	11.7	±1.3	10.9	±0.9	abc
Mannanase	NSP	2.8	±0.4	3.3	±0.4	1.6	±0.3	1.3	±0.1	1.3	±0.1	ac
	UA	0.0		0.0		0.1	±0.1	0.1	±0.0	0.2	±0.0	ac
	NS	2.8	±0.4	3.3	±0.4	1.5	±0.4	1.2	±0.1	1.1	±0.1	ac
Xylanase	NSP	1.0	±0.2	1.2	±0.4	0.9	±0.2	0.7	±0.1	0.5	±0.1	bc
	UA	0.0		0.0		0.0		0.0		0.0
	NS	1.0	±0.2	1.2	±0.4	0.9	±0.2	0.7	±0.1	0.5	±0.1	bc
Glucanase	NSP	13.9	±2.2	15.6	±4.2	15.9	±1.4	14.4	±1.9	13.9	±1.8
	UA	0.0		0.0		0.2	±0.1	0.2	±0.1	0.2	±0.1	ac
	NS	13.9	±2.2	15.6	±4.2	15.7	±2.6	14.2	±2.3	13.7	±2.0

Analyses were carried out by colorimetric analyses at 5 time points (60 and 110DAF, H: harvest, 1 M and 2 M: i.e.,1 and 2 months of cold storage) on samples released after sequential treatments with water (water-soluble, WS), pectinases (pectinase-soluble, PS), mannanases, xylanase and finally glucanase. Non-starch polysaccharides content (NSP) are expressed as a percentage of AIM dry weight, uronic acids (UA) and neutral sugars (NS) contents are expressed as a percentage of initial NSP dry weight. a, b, c: significant differences between 60 DAF and H, H and 2 M, 60 DAF and 2 M with p < 0.0001

ISSN: 1471-2229