Fig. 1

Mean MALDI-TOF MS spectra (a, d) and principal component analysis (b, c, e, f) of MS ions of annotated oligomers in the mannanase (a, b, c) and glucanase (d, e, f) digests. 60 DAF: 60 days after flowering, 110 DAF: 110 days after flowering, H: harvest, 1 M: 1 month of cold storage, 2 M: 2 months of cold storage. Nomenclature of xyloglucan oligosaccharides followed that of [131, 133] extended to account for acetyl groups noted a. In brief, it uses uppercase letters representing an individual 1 → 4 linked β -D-glucose residue and its pendant side chains. Accordingly, bare glucose residue is designated by the letter G while when branched by α -D-xylosyl residue on O-6, it is refers to X. With further extension of the branch by one β-D-galactosyl linked on xylose O-2, the trisaccharide structure formed is referred to L and when the latter is further extended by one α-L-fucose residue linked at O-2 of galactose, the structure is then referred to F. Hexose containing oligosaccharides attributed to galactoglucomannans were noted Hex. Pentose based oligosaccharides were noted Pen. These codes are extended by U, m and a when the residues are substituted by an uronic acid, a methyl and and acetyl group, respectively. The number following the structure codes denoted the number of building structures and substituent groups in the oligosaccharides (i.e., Hex3a2 corresponds to 3 hexoses and 2 acetyl groups; Pen5U1m1a1 corresponds to 5 pentose, 1 uronic acid, 1 methyl and 1 acetyl groups)