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Fig. 4 | BMC Plant Biology

Fig. 4

From: Ectopically expressed glutaredoxin ROXY19 negatively regulates the detoxification pathway in Arabidopsis thaliana

Fig. 4

a Western blot analysis to detect HA-tagged ROXY19CPYC. For comparison, a protein extract from 35S:ROXY19 line #8 was loaded on the same blot (lanes with non-relevant samples were excised). b Quantitative RT-PCR analysis of CYP81D11 in independent transgenic lines expressing either ROXY19 or ROXY19CPYC. Four-week-old soil-grown plants of the indicated genotypes were either sprayed with 0.1 mM TIBA/0.05 % DMSO or 0.05 % DMSO (mock). Leaves were harvested for RNA isolation after 10 h of treatment. Relative transcript levels were determined using UBQ5 as a reference gene. The mean values (+/−SE) obtained from four to five individually harvested plants are shown. c Growth phenotypes of the plant lines analysed in (a) and (b) on MS plates containing 1 % sucrose and 50 μM TIBA/0.025 % DMSO (lower panels) or 0.025 % DMSO (upper panels). Photographs were taken two weeks after germination

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