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Fig. 1 | BMC Plant Biology

Fig. 1

From: Tracheid cell-wall structures and locations of (1 → 4)-β-d-galactans and (1 → 3)-β-d-glucans in compression woods of radiata pine (Pinus radiata D. Don)

Fig. 1

Fluorescence micrographs of transverse sections of OW and three CWs showing lignin autofluorescence. In OW tracheid walls (a) lignin autofluorescence is strongest in the compound middle lamella (CML), the middle lamella at the cell corners (MLCC), and in the S3 layer of the secondary walls. In MCW1 (b), there is moderate autofluorescence in the middle lamella at the cell corners, and some autofluorescence in the S2L region (S2L) at the cell corners. In MCW2 (c), autofluorescence of the S2L layer at the cell corners is more evident and this layer is also fluorescent around the cell. In SCW (d) there is a highly fluorescent S2L layer all around the tracheids and there is no fluorescence of the CML. Helical cavities (HC) are present on the inner region of the S2 layer (S2i). Intercellular spaces (IS) are present in MCW 2 and SCW. Sections were from Tree 1 and the micrographs obtained using a Leica confocal microscope. Scale bar: 10 μm

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