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Table 4 Expressionlevels of four FAD2 genes in a null segregant (NG) and an OsFAD2-1RNAi line at 10, 15 and 20 DAA developmental stages

From: RNAi-mediated down-regulation of the expression of OsFAD2-1: effect on lipid accumulation and expression of lipid biosynthetic genes in the rice grain

DAA

Gene

Rice Genome Annotation

Project locus ID

NG 1 (RPKM)

NG2 (RPKM)

NG3 (RPKM)

NG (RPKM mean)

OsFAD2-1RNAi 1 (RPKM)

OsFAD2-1RNAi 2 (RPKM)

OsFAD2-1 RNAi 3 (RPKM)

OsFAD2-1RNAi (RPKM mean)

10

OsFAD2–1

LOC_Os02g48560

296.6

371.57

482.81

383.66

188.21

210.85

158.01

185.69

15

OsFAD2–1

LOC_Os02g48560

134.18

133.69

160.53

142.8

34.8

89.31

59.16

61.09

20

OsFAD2–1

LOC_Os02g48560

89.67

89.96

128.16

101.93

54.63

55.79

70.9

60.44

10

OsFAD2–2

LOC_Os07g23430

0

0

0

0

0

0

0

0

15

OsFAD2–2

LOC_Os07g23430

0

0

0

0

0

0

0

0

20

OsFAD2–2

LOC_Os07g23430

0

0

0

0

0.13

0

1.3

0.48

10

OsFAD2–3

LOC_Os07g23410

0

0

0.15

0.53

0.15

0.5

0

0.23

15

OsFAD2–3

LOC_Os07g23410

0.19

0.06

0

0

0

0

0

0

20

OsFAD2–3

LOC_Os07g23410

0.46

0.15

0.31

0

0.31

0

0

0.10

10

OsFAD2–4

LOC_Os07g23390

0

0

0

0

0

0

0

0

15

OsFAD2–4

LOC_Os07g23390

0

0.22

0

0.07

1.18

0.36

0

0.51

20

OsFAD2–4

LOC_Os07g23390

0

0

0

0

0

0.82

1.87

0.90

  1. DAA-days after anthesis