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Fig. 3 | BMC Plant Biology

Fig. 3

From: Comparative proteomics illustrates the complexity of drought resistance mechanisms in two wheat (Triticum aestivum L.) cultivars under dehydration and rehydration

Fig. 3

2-DE gel analysis of proteins extracted from leaves of Xihan No. 2 during dehydration and rehydration. Equal amounts (900 μg) of proteins were separated on pH 3–10 IPG strips (17 cm, linear) in the first dimension and by SDS-PAGE on 12 % polyacrylamide gels in the second dimension. The gels were visualized by CBB staining. Three replicate CBB-stained gels for control, dehydration treatments (18 h, 24 h and 48 h) and rehydration treatment (R24 h) (Additional file 1: Figure S3) were computationally combined using PDQuest v8.0.1 software, respectively. Protein spots indicated with numbers were identified by MALDI-TOF/TOF MS. The identified spots were numbered in accordance with Additional file 6: Table S4. a 2-DE protein profile for control; (b-e) 2-DE protein profile for dehydration treatments (18 h, 24 h and 48 h) and rehydration treatment (R24 h), respectively

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BMC Plant Biology

ISSN: 1471-2229

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