Fig. 7From: BnPME is progressively induced after microspore reprogramming to embryogenesis, correlating with pectin de-esterification and cell differentiation in Brassica napus Temporal patterns of esterified and non-esterified pectins during gametophytic development and microspore embryogenesis. Immuno-dot-blot assays at different developmental stages: vacuolated microspore, differentiated pollen (gametophytic development), early proembryo, globular, torpedo and cotyledonary embryo (microspore embryogenesis). Ponceau Red staining for total proteins and immuno-dot blot of the same strip is shown for each antibodyBack to article page