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Fig. 2 | BMC Plant Biology

Fig. 2

From: BnPME is progressively induced after microspore reprogramming to embryogenesis, correlating with pectin de-esterification and cell differentiation in Brassica napus

Fig. 2

Expression analysis of BnPME gene by RT-PCR during gametophytic development and microspore embryogenesis. Semiquantitative RT-PCR representative agarose electrophoresis gels of the amplification products. Histogram expresses relative changes in the PME bands intensities at different developmental stages: vacuolated microspore, differentiated pollen (gametophytic development), early proembryo, globular embryo, cotyledonary embryo (microspore embryogenesis). Columns represent means of mRNA expression of BnPME ± SE (bars) of three independent experiments with three replicates each, in arbitrary units (a.u.). Asterisks indicate significant differences with the vacuolated microspore (Student’s t-test at P ≤ 0.05)

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