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Fig. 4 | BMC Plant Biology

Fig. 4

From: Identification of candidate genes related to calanolide biosynthesis by transcriptome sequencing of Calophyllum brasiliense (Calophyllaceae)

Fig. 4

a Maximum likelihood phylogenetic tree depicting the relationship between the different C. brasiliense (blue) and Arabidopsis (green) PAL family members. Protein sequences were aligned with MUSCLE [69], and phylogenetic analyses were performed in maximum likelihood framework with SeaView v4.0 software [39]. b Primary protein structures in which the aromatic amino acid lyase conserved domain (PF00221) are represented by gray boxes. c Matrix of the percentage identities between the aligned protein sequences of PAL family members from C. brasiliense and Arabidopsis. d Structural model superposition of PAL proteins from C. brasiliense [UN01310 (pink), UN02730 (yellow) and UN03680 (cyan)]. The available structure of PAL protein from Petroselinum crispum in the Protein Database (PDB entry 1w27.1) was used as a template. The homology modeling was carried out using the SWISS-MODEL program and SWISS-PDB viewer v4.1.0 ([73, 75]; http://swissmodel.expasy.org/) was used to superpose the C. brasiliense PAL proteins

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