Fig. 1

a Hierarchical clustering of C. brasiliense preferentially expressed unigenes based on RPKM values. The log₂ RPKM values are presented with varying colors. The darker brown represents higher expression values, and the gray in the heat map lowest values. b RT-qPCR-based expression of eight-selected C. brasiliense preferentially expressed unigenes based on expression levels across organs (leaves, stem, and roots). Expression levels are given as 40-ΔCT, where ΔCT is the difference in threshold cycle number of the respective gene and the reference UN18770 (homolog to AT4G05050 which codify to UBQ11 on Arabidopsis); the number 40 was chosen because the PCR run stops after 40 cycles and a constant value was required as calibrator (see as example [76]). The results are shown as the averages ± SE of three biological replicate c RNA-Seq expression levels measured as reads per kb per million of reads (RPKM)