Fig. 6
Molecular analysis of representative transplastomic plants containing the edited cp genome. Southern blot containing Hind III digests of DNA from indicated lines probed with a aadA and b rbcL. c PCR analyses on DNA from shown lines using primers against aadA, gusA and petB genes. MW standards and band sizes are indicated. d Sequence of A3Eα rbcL gene showing position of deleted guanine (arrowed). e Maps of edited and iDR intermediate cp genomes showing Sac I (S) and Not I (N) sites. f Not I plus Sac I digests from the indicated lines probed with gusA. Bands from edited and iDR intermediate cp genomes are indicated