Fig. 2

WT (top) and mutant (bottom) C-terminal Rubisco LS sequences. Deletion of a guanine in the WT rbcL sequence (top) changes aspartic acid residue 473 and all subsequent amino acids in the extended C-terminus of the encoded Rubisco LS protein (bottom). The ATG start codon of gusA and TAG stop codon of the mutant rbcL gene are separated by ten nucleotides. The sequence between rbcL and gusA is a synthetic linker sequence that contains a Hind III site. A predicted RNA secondary structure [41] containing the gusA start codon is shown