Skip to main content
Fig. 1 | BMC Plant Biology

Fig. 1

From: Seamless editing of the chloroplast genome in plants

Fig. 1

Two-step editing scheme. a The vector contains an imperfect direct repeat (iDR) of a partial rbcL sequence (arrows) separated by a selectable marker (SM). The right iDR contains a point mutation (*). Homologous recombination mediates integration via the left (L) and right (R) targeting arms and gives rise to the intermediate cp genome shown. For simplicity recombination between the right iDR and rbcL gene is not shown (see Fig. 3). Recombination between the repeated sequences excises the SM and gives rise to the edited cp genome containing a point mutation. b and c show variations that enable alternative edited products. In b a gene of interest (GOI) is inserted into the cp genome using a vector with perfect direct repeats (DR). In c a scheme for inserting a mutation and a GOI is shown. In this example the GOI overlaps a mutant rbcL gene. Translation coupling between the upstream rbcL gene and the overlapping GOI will result in GOI expression in the edited cp genome but not in the intermediate where the partial upstream rbcL gene forming the iDR is not translated. Not to scale

Back to article page