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Fig. 8 | BMC Plant Biology

Fig. 8

From: Cold regulation of plastid ascorbate peroxidases serves as a priming hub controlling ROS signaling in Arabidopsis thaliana

Fig. 8

sAPX and tAPX protein levels in the lag-phase after LTC and STC. The proteins were detected with an antibody against the stromal part of Arabidopsis tAPX, which recognizes chloroplast and most cytosolic APXs. a The band intensities were quantified on 3–4 blots after luminometric detection. The data were analyzed by ANOVA (Student t-test; p < 0.05, n = 3–4). Significant differences between primed and unprimed plants are labeled with an asterisk. Data on primed plants are shown in blue, those obtained with naïve plants in white. b Examples of luminograms obtained by Western-Blotting with the APx antibody in STC- and LTC-treated plants and a digital photo of the PonceauS stained rbcL on the same membranes prior to the incubation in the antibody solution

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