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Fig. 6 | BMC Plant Biology

Fig. 6

From: The functional divergence between SPA1 and SPA2 in Arabidopsis photomorphogenesis maps primarily to the respective N-terminal kinase-like domain

Fig. 6

SPA1 and SPA2 similarly interact with COP1 in dark-grown vs. light-grown seedlings. Co-immunoprecipitation of COP1 by SPA1-HA and SPA2-HA in the 4-day-old spa1 spa2 spa3 seedlings expressing SPA1-HA and SPA2-HA proteins under the control of the native SPA2 promoter. Seedlings were grown in darkness and were subsequently transferred to 0.05 μmol m−2 s−1 FR or R for 2 h or 50 μmol m−2 s−1 B for 1 h. Proteasome inhibitor was added to prevent degradation of SPA proteins in blue light. SPA1-HA and SPA2-HA proteins were immunoprecipitated using α-HA beads. Col-0 was used as a negative control. α-HA antibody was used to detect SPA-HA proteins. α-COP1 antibody was used to detected COP1. Equal amount of total protein was used as inputs

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BMC Plant Biology

ISSN: 1471-2229

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