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Fig. 4 | BMC Plant Biology

Fig. 4

From: The functional divergence between SPA1 and SPA2 in Arabidopsis photomorphogenesis maps primarily to the respective N-terminal kinase-like domain

Fig. 4

The SPA1 N-terminal domain provides the higher stability to the chimeric DS_122-HA protein in the light. a-c SPA-HA protein levels in 4-day-old T3 homozygous transgenic spa1 spa2 spa3 mutant seedlings grown in darkness (D) and subsequently transferred to 0.35 μmol m−2 s−1 FR (a), 0.01 μmol m−2 s−1 R (b) or 0.1 μmol m−2 s−1 B (c) for 30 min. SPA-HA was detected using an α–HA antibody. HSC70 levels served as a loading control. Numbers indicate independent transgenic lines

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BMC Plant Biology

ISSN: 1471-2229

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