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Fig. 3 | BMC Plant Biology

Fig. 3

From: The functional divergence between SPA1 and SPA2 in Arabidopsis photomorphogenesis maps primarily to the respective N-terminal kinase-like domain

Fig. 3

WD-repeat domain is involved in the functional divergence of SPA1 and SPA2. a Schematic representation of chimeric SPA1/SPA2 domain swap DS_112-HA, DS_121-HA and DS_211-HA constructs. All chimeric constructs were expressed in a spa1 spa2 spa3 mutant background under the control of the SPA2 promoter and fused with a C-terminal HA tag. NLS indicates the location of a predicted nuclear localization sequence. b Phenotype of 4-day-old spa1 spa2 spa3 seedlings expressing DS_112-HA, DS_121-HA and DS_211-HA domain swap constructs. Representative T2 seedlings are shown. Seedlings were grown in darkness or in 0.05 μmol m−2 s−1 FRc, 0.01 μmol m−2 s−1 Rc or 0.05 μmol m−2 s−1 Bc for 4 days. Numbers indicate independent transgenic lines

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