Fig. 2

SCAP1 protein differentially accumulates in plant tissues. (a-e) Confocal images of pro35S:SCAP1-YFP (35S:SCAP1-YFP) and, (f-i), pro35S:YFP (35S:YFP) plants at different stages. (a, f) Whole seedling. (b, g) First leaf primordia (5 das). (c, h) Epidermis of the first leaf primordia (5 das). (d, i) GCs in a cotyledon (7 das). (e) Epidermis of cotyledons (7 das). Asterisks mark epidermal cells, arrows heads mark dividing cells. Images a, b, f and g are the sum of all the z stacks obtained across the entire thickness of the sample. Images c-e and h-i are the sum of those z stacks corresponding to the epidermis. Bars = 1 mm (a, f); 50 μm (b-e, g-i). SCAP1-YFP/YFP protein signal is shown in yellow, autofluorescence (chlorophyll) in red. (j) Cotyledon epidermis of GUS stained proSCAP1:GUS-GFP plants (7 das). Bars = 100 μm