Identification of single nucleotide polymorphism markers associated with resistance to bruchids (Callosobruchus spp.) in wild mungbean (Vigna radiata var. sublobata) and cultivated V. radiata through genotyping by sequencing and quantitative trait locus analysis

Table 2 Validation of markers dCAPS2, dCAPS3, CAPS1 and CAPS12 in the F3 generation of V2802 × NM94

Family number	% damage	dCAPS2	dCAPS3	CAPS1	CAPS12	Family number	% damage	dCAPS2	dCAPS3	CAPS1	CAPS12
9	0	RR	RR	RR	RR	59	50.0	H	H	H	H
14	0	RR	RR	RR	RR	46	53.3	H	H	H	H
24	0	RR	RR	RR	RR	56	56.7	H	H	H	H
30	0	RR	RR	RR	RR	27	63.3	H	H	H	H
36	0	RR	RR	RR	RR	18	26.7	SS	SS	SS	SS
50	0	RR	RR	RR	RR	16	33.3	SS	SS	SS	SS
52	0	SS	H	RR	RR	84	36.7	SS	SS	SS	SS
69	0	RR	RR	RR	RR	55	40.0	SS	SS	SS	SS
89	0	RR	RR	RR	RR	33	48.2	SS	SS	SS	SS
93	0	RR	RR	RR	RR	47	50.0	SS	SS	SS	SS
72	0	RR	RR	RR	RR	66	50.0	SS	SS	SS	SS
51	6.7	RR	RR	RR	RR	42	53.3	SS	SS	SS	SS
1	10.00	RR	RR	RR	RR	96	60.0	SS	SS	SS	SS
15	16.7	RR	RR	RR	RR	71	60.0	SS	SS	SS	SS
29	20.0	RR	RR	RR	RR	100	60.0	SS	SS	SS	SS
65	23.3	RR	RR	RR	RR	37	65.2	SS	SS	SS	SS
82	23.3	RR	RR	RR	RR	86	70.0	SS	SS	SS	SS
90	23.3	RR	RR	RR	RR	70	70.0	SS	SS	SS	SS
43	23.3	RR	RR	RR	RR	91	73.3	SS	SS	SS	SS
6	33.3	RR	RR	RR	RR	98	73.3	SS	SS	SS	SS
13	36.7	RR	RR	RR	RR	102	76.6	SS	SS	SS	SS
48	36.7	RR	RR	RR	RR	54	76.7	SS	SS	SS	SS
67	40.0	RR	H	H	RR	83	80.0	SS	SS	SS	SS
10	13.3	H	H	H	H	7	80.0	SS	SS	SS	SS
41	23.3	H	H	H	H	94	80.0	SS	SS	SS	SS
85	26.7	H	H	H	H	4	80.0	SS	SS	SS	SS
40	30.0	H	H	H	H	32	82.9	SS	SS	SS	SS
57	40.0	H	H	H	H	76	83.3	SS	SS	SS	SS
99	40.0	H	H	H	H	31	83.3	SS	SS	SS	SS
26	46.7	H	H	H	H	20	86.7	SS	SS	SS	SS
63	53.3	H	H	H	H	61	86.7	SS	SS	SS	SS
62	70.0	H	H	H	H	58	86.7	SS	SS	SS	SS
60	50.0	H	H	H	H	53	90.0	SS	SS	SS	SS
81	90.0	RR	RR	RR	SS	97	90.0	SS	SS	SS	SS
75	13.3	H	H	H	H	11	93.3	SS	SS	SS	SS
77	13.3	H	H	SS	SS	5	93.3	SS	SS	SS	SS
35	16.7	H	H	H	H	21	93.3	SS	SS	SS	SS
23	16.7	H	H	H	H	17	93.3	SS	SS	SS	SS
49	23.3	H	H	H	H	19	93.3	SS	SS	SS	SS
74	23.3	H	H	H	H	73	93.3	SS	SS	SS	SS
101	36.7	H	H	H	H	38	96.7	SS	SS	SS	SS
44	40.0	H	H	H	H	80	100.0	SS	SS	SS	SS
39	43.3	H	H	H	H	34	100.0	SS	SS	SS	SS

RR, SS: homozygote resistance and susceptibility allele, respectively. H: heterozygote. %damage: Percent of damaged seed in bruchid resistance assays. Loci where the 4 tested markers did not co-segregate are printed in bold

ISSN: 1471-2229